Alina Pilipenco 1,2, Milan Houska1, Michala Forinová1,2, Vera Vizelka3, Guruprakash Subbiahdoss4, Erik Reimhult4, Hana Vaisocherová-Lísalová1 

1 FZU - Institute of Physics of the Czech Academy of Sciences, Na Slovance 1999/2, 180 00 Prague, Czech Republic 
2 Institute of Physics, Faculty of Mathematics and Physics, Charles University, Ke Karlovu 3, Prague, 121 16, Czech Republic 
3 Faculty of Natural Sciences, Charles University, Albertov 6, 128 00 Prague, Czech Republic
4 Institute of Colloid and Biointerface Science, BOKU University, Muthgasse 11, A-1190 Vienna, Austria

Figure 1. The FTIR spectra of terpolymer bushes before and after activation with (a) NHS/EDC and (b) sulfo-NHS/EDC activation, showing the spectral changes over 150 min. 

Figure 2. The time dependence of the degree of surface activation for sulfo-NHS/EDC (black squares) and NHS/EDC (red circles) assessed from the reaction conversion over time as quantified by FT-IRRAS.

Table 1. ADSORBED MASS FROM SERUM-RICH MEDIUM (QCM-D).

Fig. 3. SaOS-2 cell attachment and spreading on terpolymer brushes RGD-functionalized via NHS/EDC or sulfo-NHS/EDC. (a) Phase-contrast images show time-dependent SaOS-2 cell adhesion, spreading, and proliferation on terpolymer-coated surfaces (scale bars: 100 μm) (b) Fluorescence microscopy images of SaOS-2 cells stained for actin (red) and nuclei (blue) (scale bars: 50 μm). (c) The percentage of cell surface coverage after 48 h of incubation on different surfaces.

More details concerning the data can be provided on request.